2and and are representative of at least two independent experiments with = 6 mice per group

2and and are representative of at least two independent experiments with = 6 mice per group. effects comparable to the unmodified antibody but did not result in liver inflammation. Moreover, it efficaciously synergized with both PD-1 blockade and adoptive T-cell therapy. Surprisingly, minimal active Pb-Tx reached tumor-draining lymph nodes, and regional lymphadenectomy did not abrogate antitumor efficacy. By contrast, S1P receptorCdependent recirculation of T cells was absolutely required for efficacy. The preferential cleavage of the anti-CD137 Pb-Tx by tumor proteases offers multiple therapeutic opportunities, including neoadjuvant therapy, as shown by Inulin experiments in which the Pb-Tx is given prior to surgery to avoid spontaneous metastases. Immunotherapy based on blocking T-cell coinhibitory receptors has revolutionized cancer clinical management with the advent of antiCPD-(L)1 and antiCCTLA-4 checkpoint inhibitors (1). Immunostimulatory agonist antibodies targeting costimulatory targets such as CD137 (4-1BB), OX40, ICOS, GITR, or CD27 are lagging behind in clinical development for a variety of reasons (2, 3). In the case Inulin of agonist anti-CD137 monoclonal antibodies (mAb), the immunotherapeutic effects in mouse models are impressive (4), especially when combined with antiCPD-(L)1 (5, 6), interleukin-12 (7), or adoptive T-cell therapy (ACT) (8, 9). The mechanism of action mainly relies on CD8 T-cell costimulation and reinvigoration of dysfunctional tumor-infiltrating lymphocytes (8, 10). In the clinic, the strong CD137 agonist Urelumab underwent several clinical trials (11) but was limited to subtherapeutic doses by frequent and serious liver inflammation above 0.3 mg/kg (11). Another anti-CD137 mAb, Utomilumab (12), shows weak intrinsic agonist activity and could be safely dosed up to 10 mg/kg but without consistent evidence for clinical activity as a monotherapy (13). Many attempts are ongoing in the clinic to safely target CD137 costimulation to the tumor while preserving liver safety (14, 15). These include bispecific antibodies (16) and tumor-targeted constructs containing trimeric natural ligand (CD137L) (17). Here, we report the immune and therapeutic effects of an agonist anti-CD137 antibody prodrug called a Probody therapeutic (Pb-Tx) (18, 19). The term Probody is a United StatesCregistered trademark of CytomX Therapeutics to refer to a new class of recombinant, proteolytically activated antibody prodrugs. The anti-CD137 Pb-Tx is designed to be activated to exert agonist activity on CD137 only in the tumor when cleaved by tumor-associated proteases. Results A Tumor ProteaseCCleavable Anti-CD137 Pb-Tx. To mediate tumor-associated activation of an anti-mouse CD137 mAb, a Pb-Tx construct was made based on an IgG1 murine version of the well-described agonist antibody 1D8 Rabbit polyclonal to AFF3 (4). The N terminus extension of the light chains of this antibody was engineered with a protease-sensitive linker (18, 19) designed to be cleaved by proteases active in the tumor microenvironment (and are representative of at least three independent experiments in triplicate wells. 1D8 Anti-CD137 Pb-Tx Mediates Antitumor Effects with Reduced Liver Inflammation. In mouse models, potent antitumor immunotherapy based on systemic administration of anti-CD137 agonist mAb (4) is accompanied by augmented CD8 T-cell liver infiltration and increased transaminases (22C24). Intraperitoneal administration of both the murine IgG1 version 1D8 mAb or the Pb-Tx leads to consistent rejection of CT26-derived tumors engrafted for 6 d in BALB/c mice prior to therapy onset, which grew unaffected after treatment with control isotypeCmatched irrelevant IgG1 (Fig. 2and and are representative of at Inulin least two independent experiments with = 6 mice.