Following, 15 to 50 g of proteins was fractionated on the SDS-polyacrylamide gel, used in a polyvinyl difluoride membrane, blotted with the correct principal antibody [dilutions were 1:1,000 anti-Glut3 (60 kDa), 1:800 for anti-HKII (100 kDa), 1:1,000 anti-cleaved caspase 3 (17/19 kDa), 1:1,000 anti-PARP (116/89 kDa), and 1:10,000 for anti–actin (45 kDa)], and subsequently with horseradish peroxidase-conjugated donkey anti-goat (1:20,000), goat anti-rabbit (1:10,000), goat anti-mouse (1:10,000)

Following, 15 to 50 g of proteins was fractionated on the SDS-polyacrylamide gel, used in a polyvinyl difluoride membrane, blotted with the correct principal antibody…