The mouthwash samples, which will be collected in physiological solution, will be centrifuged, resuspended, and seeded, in duplicate, in Petri dishes containing MacConkey agar (Difco, Detroit, MI, USA) for the isolation of enterobacteria/pseudomonas. and temporal muscle tissue), physiological sleep variables, and salivary guidelines in adult individuals with DS. Methods The individuals with DS will become randomly selected and divided into Cd14 three organizations (DS-NMES, DS-MA, and DS-OAm) with a minimum of 10 individuals in each group. A thermosensitive microchip will become inlayed in the OAm to record its compliance. The restorative effects on masticatory muscle mass function will become investigated through electromyography, a caliper, and a force-transducer device; the sleep variables, in turn, will become evaluated by means of polysomnography. The physicochemical and microbiological properties of the saliva will also be analyzed, including the salivary circulation, viscosity, buffer capacity, cortisol levels (susceptibility to mental and/or physical stress), and levels (risk of aspiration pneumonia) in these individuals. The methods identified for this study will become carried out prior to and after 2?months of the recommended treatments. Discussion The primary outcomes would be the improvement and/or reestablishment of the function of masticatory muscle tissue and the physiological sleep variables with this target public since individuals with DS generally present generalized muscular hypotonia and dysfunction of the oropharyngeal musculature. As a secondary outcome indication, the impact of the applied treatments (NMES, MA, and OAm) within NMS-E973 the salivary microbiological and physicochemical properties in DS individuals will also be assessed. Furthermore, the compliance of OAm utilization will become measured through a thermosensitive microchip. Trial sign up Registro Brasileiro de Ensaios Clnicos, RBR-3qp5npRegistered on 20 February 2018. Electronic supplementary material The online version of this article (10.1186/s13063-019-3300-0) contains supplementary material, which is available to authorized users. varieties, the saliva sample must be analyzed within a maximum period of 3?h. Analysis of the physical salivary properties of the saliva Method for measurement of the saliva circulation After comfortable and postural adaptation of the individuals on the chair, the saliva will become stimulated through a gustatory sialagogue, and then continually collected for 5?min by means of NMS-E973 a Falcon-type sterile tube which will be coupled to an aspirating pump. The saliva circulation (SF) will become calculated from the method: SF?=?total salivary volume / salivary collection time. The research ideals are explained in the study by Gomes et al. . Method for measurement of the salivary viscosity Saliva viscosity (consistency) will be considered as this physical house strongly helps in regular swallowing. In light of this, the viscosity will become evaluated by means of an Ostwald viscometer, model Cannon-Fenske Program, size 350, internal capillary having a caliper of 1 1.52 (Hermex Indstria e Comrcio de Vidros em virtude de Laboratrio, S?o Paulo, Brazil). Research ideals (centipoise) are ?1.5 cp for nonviscous saliva and??1.5 cp for viscous saliva. In order to standardize the screening temperature, the viscometer will become vertically situated from the L tube inside a thermal bath at 37?C for 15?min. A sample of 5?ml of saliva samples will then be inserted into the N tube of the Ostwald viscometer, using a hypodermic syringe. Concomitantly, aspiration will become carried out by inserting the pear into the L tube opening to push the saliva toward the E mark of the NMS-E973 C bulb. Thereafter, the vacuum will become released and the saliva outflow time, between the E and F marks, will become clocked. This strategy is definitely explained in the studies by Almeida et al.  and Pedersen et al. . Analysis of the chemical salivary properties of the saliva Method for measurement NMS-E973 of the buffer capacity In this test, a 1?ml sample of saliva will be transferred to NMS-E973 a sterile Falcon-type tube. Next, 3?ml of hydrochloric acid (0.005?N) will be added to this sample. The tube will be lightly shaken and allowed to rest for 10?min. Immediately thereafter, the pH will become measured with the aid of a.